Regulatory

Part:BBa_K112400:Design

Designed by: Christina Brown   Group: iGEM08_UC_Berkeley   (2008-10-20)

Promoter for grpE gene - Heat Shock and Ultrasound Sensitive


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pBca1256-K112400

Biobricking of promoter for grpE protein 
 PCR grpE-F and grpE-R on MG1655 	  (129 bp, EcoRI/BamHI)
 Sub into pBca1256	                  (EcoRI/BamHI 910+2472bp, L)
 Product is pBca1256-K112400
 ----------------------------
cb0001-grpE-F Forward Biobricking    cgataGAATTCatgAGATCTccgaggtccttgttgcgaag
cb0002-grpE-R Reverse Biobricking    cgtagGGATCCcgcattcatctcgctaacttc



Source

cloned from E. coli MG1655 genome

References